11/1/2022 0 Comments Protein scaffold minimal![]() ![]() Thereby the same protocol is used for all different variants of MSP: 1.įreshly transform the selected MSP expression plasmid (Addgene) in BL21 (DE3) star cells, streak them out on an agar plate containing kanamycin and incubate the plate overnight at 37☌. coli cells and purified via the N-terminal His 6-tag ( Denisov et al., 2004). The MSP can be produced by conventional expression in E. However, the mechanistic details whereby many of the scaffold proteins promote pathway activation remain to be elucidated. Additional scaffold proteins have been identified that participate in the activation of p38 and ERKs. Additionally, WDR62 (WD repeat domain 62) has been implicated in noncanonical activation of JNK via association with JNK and MKK7. In response to stress resulting from X-rays and genotoxic drugs, RACK1 (Receptor for Activated C Kinase 1) has been shown to bind and activate JNK1 and MTK1. Following cleavage by caspase-3, the C-terminal domain of GRASP1 is released to promote activation of JNK. In neurons, GRASP-1 (GRIP1-associated protein 1) binds both JNK1 and MEKK1. In 2016, another MKP, DUSP22, was reported to interact with ASK1, MKK7, and JNK1 to promote JNK activation independent of its phosphatase activity. DUSP19 physically interacts with MKK7 and indirectly regulates JNK. ĭUSP19 (dual-specificity phosphatase 19) is the first MAPK phosphatase (MKP) identified as a scaffold protein. Crk II activates HPK1 (hematopoietic progenitor kinase 1) and SEK1-dependent activation of the JNK pathway, by recruiting JNK1 to a p130Cas multiprotein complex. β-Arrestin-2 contains a MAP kinase docking site and mainly functions as a scaffold protein in activation of JNK3, by tethering ASK1, SEK1, and JNK3. JIP1 recruits DLK, MKK7, and JNK as a complex, whereas JIP3 binds SEK1 and MEKK1 to stimulate SEK1, leading to JNK3 activation. Scaffolds JIP1 (JNK-interacting protein 1) and JIP3 recruit several different activators and JNKs. POSH complexes with and sequentially stimulates RAC1, MLKs, SEK1, MKK7, and JNKs. The scaffold proteins POSH (plenty of SH3s) promote JNK pathway activation by directly interacting with GTP-bound RAC1. In melanoma cells, filamin was found to interact with SEK1 as a scaffold protein, in response to TNF-α stimulation. Several scaffold proteins have been identified that bind to JNKs and upstream activators. Scaffold proteins play key roles in providing a platform for signaling molecules to assemble, promoting the localization of signaling molecules at specific sites and coordinating positive and negative feedback signals for pathway regulation. Johnson, in Targeting Cell Survival Pathways to Enhance Response to Chemotherapy, 2019 4.2.3 The Role of Scaffold Proteins ![]()
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11/1/2022 0 Comments Cabin fever streaming![]() ![]() So many of us artists are in precarious financial positions even in regular times, and the COVID-19 pandemic only exacerbates the situation. Maybe you can find them via social media. Perhaps they have websites with donation links or contact info. If you are enjoying movies on this playlist and are in a position to be able to spare some money, I hope that you will consider reaching out to individual artists whose work you are watching and find out how you can donate money to them in appreciation of their creative contributions. **IMPORTANT NOTE: PLEASE SUPPORT ARTISTS!!** I love it!! I do hope that the original goal of feeding emotional needs and fostering virtual connectivity continues to be the salient purpose of the document. The list has morphed some, with some great new categories having been added to the mix. ![]() The initial intention of this list was to gather some experimental films together based on moods one might be experiencing while being cooped up. Please note that I have a lot going on in addition to maintaining the playlist, so I may not be able to make additions right away. If there are experimental films or works of video art that you’d like to suggest for the playlist, you can use this form. #CABIN FEVER STREAMING DOWNLOAD#If you are here to access the list in more of a reading/learning capacity, please download it (File > Download > ) and then close this tab *OR* download a recent version of it from Hi, everyone! Welcome to this growing and morphing collaborative resource! A note upfront that google limits the number of people accessing it to 100 at any given time, and traffic has been growing and growing, so I have had to make this list view-only (at least for now) and change how people can add new films to the list (see below for details). ![]() 11/1/2022 0 Comments Paper protractor![]() For example, if you counted 20° from the dotted line, the reflex angle measures 180 + 20 which is 200°. Place the protractor on the vertex of the base line, and draw a dotted line for 180°.įlip the paper, keep the protractor on the vertex, and measure from the dotted line to the angle line. Paper protractor how to#How to Measure Angles Exceeding 180° Using a Semicircular Protractor Then, flip the paper, and you have your desired angle exceeding 180° without using a circular protractor. So, 260° will be an additional 80° from 0.Īfter you’ve marked the said angle, draw a line connecting the vertex and the marked point. For instance, if you wish to draw an angle measuring 260°, begin counting from 180 to 260. Considering the dotted line as your base line, and 0° as 180°, mark the desired angle. Now flip the paper, and hold the protractor once again on the marked vertex. Once measured, draw a dotted line from the vertex to the marked point. Additionally, we’ll also teach you to draw or measure angles more than 180° using a regular semicircular protractor. Let’s begin with learning to draw an angle using a protractor, and also learn how to measure already drawn angles. ![]() However, a semicircular protractor is also capable of measuring angles up to 360°, even though it does not have the respective markings. If semicircular protractors measure angles up to 180°, how much can a circular protractor measure? Simple! 360°. These are mostly semicircular, and can be used to draw or measure angles up to a maximum of 180°. Protractors are widely used in schools to help students learn geometry. ![]() ![]() The latter are popularly referred to as radian-scale protractors and are used for complex, mechanical figures especially in the field of engineering or architecture. Most protractors measure angles in degrees, whereas a few also measure them in radians. ![]() 11/1/2022 0 Comments Ring of visibility osrs![]() Go talk to him and he will tell you about the god wars and eventually ask you if you want to help and then he will ask you for some material to make a Scrying Glass. Then talk to him about the 4 gems (the lowest talk option, and he will say Crystals not Gems) and he will tell you to talk to Eblis (eastern side of the camp, just south of the general store near the south side of a building there). Go to the camp and buy a beer (Bandit's Brew) from the bartender for 650 coins. The Bandits are not affected by items aligned with the other gods. They are only level 78, but if they swarm you they can be very annoying and potentially dangerous if you have low-level gear. If you do wear such items, the Bandits will attack you. Note: Before going to the Bandit Camp, make sure you aren't wearing anything related to Saradomin or Zamorak, like Holy or Unholy symbols or the God Capes and Staffs. Agree and he tells you to go search around the Bandit Camp to the south. Return the translated notes to the Archaeologist and he says it confirms what he thought and that if you help him he'll chip you in for part of the reward. Bank and get 650 coins (plus some for the magic carpet if needed), 12 Magic Logs, 6 Steel Bars, 6 Molten Glass, 1 Blood Rune, some Ashes, some Normal Bones, and Charcoal, note all of the items except the Charcoal. Talk to him again and he will give you the translated notes. Talk to Balando with the notes and he looks at them. If you have completed all the quests required than he will tell you that he thinks there is a treasure in the desert and he wants you to take some notes to the Terry Balando, the Archaeological Expert at the Exam Center. If you have not completed all of the quests that are required for this quest than he will just be 'lost in his thoughts'. To start this adventure you must talk to the Archaeologist right beside the camp's Magic Carpet. The best way to do this is to use the Shantay Pass carpet to get there for 200 coins. Items: 1x Desert Robe, 1x Desert Shirt, 12-20x Prayer Potion, 1x Ring of Charos (a), 3-5x WaterskinĪs rumors abound you must have heard them and being interested in adventures like any good adventurer you will want to go off and find this treasure.Quests: A Fairy Tale Part II: Cure a Queen, Eadgar's Ruse, Enakhra's Lament, Garden of Tranquillity, Heroes' Quest, In Search Of the Myreque, Nature Spirit, The Feud.Skills: 38 Herblore, 43 Prayer, 60 Magic.Items: 1x Ashes, 1x Blood Rune, 1x Bones, 1x Cake or Chocolate Cake, 1x Charcoal, 1x Climbing Boots, 650x Coins, 1x Face Mask/ Gas Mask/Slayer Helmet/ Masked Earmuffs, 1x Garlic, 1x Ice Gloves, 5-50x Lockpick, 12x Magic Logs, 6x Molten Glass, 1x Pestle and Mortar, 1x Silver Bar, 1x Spice (excludes Gnome Spice), 1x Spiked Boots, 6x Steel Bar, 1x Tinderbox.Quests: Priest in Peril, Temple of Ikov, The Digsite, Tourist Trap, Troll Stronghold, Waterfall Quest.Skills: Ability to defeat 5 Ice Trolls (level 128), Dessous (level 120), Kamil (level 126), Fareed (level 120) and Damis (level 124), 10 Slayer, 50 Firemaking, 50 Magic, 53 Thieving.Begin by speaking to the Archaeologist at the Bedabin Camp. ![]() ![]() ![]() ![]()
11/1/2022 1 Comment J2k recruiting![]() ![]() Previous experience managing your own caseloads and making autonomous decisions about patient care are necessary. ![]() The candidate must hhave over five years experience working as a Nurse Practitioner.
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